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Ultimate pH values and bacteriological condition of meat and stress metabolites in blood of transported reindeer bulls

Posted on 2013 by stuartray

The Stomacher® 400 was used to blend reindeer meat for two minutes.

The study found that at slaughter, there was no difference in ASAT activity, urea and Cortisol concentrations between the two transported groups. However, the plasma ASAT activity and urea concentrations at slaughter were significantly lower in the non-transported group. In both transport groups, the plasma Cortisol concentrations increased during loading onto and unloading from the lorry. Abomasal lesions were observed in all treatment groups. It was concluded that reindeer showed an acute stress response to manual handling and transport.

food microbiology meat quality stress metabolites reindeer stomacher 400

Influence of variations in methodology on populations of Listeria monocytogenes recovered from lettuce treated with sanitizers

Posted on 2013 by stuartray

Stomaching, homogenizing, or stomaching followed by homogenizing lettuce treated with sanitizers resulted in recovery of similar numbers of L. monocytogenes, indicating that stomaching and homogenizing are equivalent in extracting cells.

food microbiology sanitizer evaluation lettuce listeria monocytogenes stomacher 400

Human wharton’s jelly cells-isolation and characterization in different growth conditions

Posted on 2013 by stuartray

The enzyme solution with tissue is taken out and put in the stomacher strainer bags with inner tissue filter at the rate of 25 ml of enzyme solution per bag. The air in the bag is removed to prevent the bag from rupturing and the bag is sealed at least twice at the top using a heat sealer. The bag is placed in the stomacher 400 Circulator and the stomacher is run at 150 rpm for 10 minutes at 37˚C.

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Measuring bacterial load and immune responses in mice infected with Listeria monocytogenes

Posted on 2013 by stuartray

The Stomacher® 80 was used to test tissue from mice. Place the Stomacher bag into the Stomacher, and run the Stomacher on high speed for 10 minutes. Mix the homogenate in the Stomacher bag.

clinical microbiology mouse spleen liver listeria monocytogenes stomacher 80

Biomonitoring by combination of immunomagnetic separation and direct epifluorescence filter technique

Posted on 2013 by stuartray

The Stomacher® 400 is used to support DEFT (Direct Epifluorescence Filter Technique).

Dried apricots and figs were used in the experiments. 10g of fruits were mixed with 90mL of phosphate buffered solution (PBS) in a ´400´ Closure Bag and homogenised in a Stomacher 400 device for 10 minutes.

food microbiology dynabead direct epifluoresence apricot stomacher 400

Enhanced Immunogenicity to Mycobacterium tuberculosis by Vaccination with an Alphavirus Plasmid Replicon Expressing Antigen 85A

Posted on 2013 by stuartray

The Stomacher® 80 was used to blend lungs and spleens of sacrificed animals. The study found that even with the optimum dose of the vaccine there was no further or enhanced protection against m.b tuberculosis.

clinical microbiology plasmid DNA vaccination lung spleen mycobacterium tuberculosis stomacher 80

Optimization of Periprosthetic Culture for Diagnosis of Propionibacterium acnes Prosthetic Joint Infection

Posted on 2013 by stuartray

Tissue specimens were homogenized in 3 ml of sterile saline using a Seward Stomacher 80, and the sterility of each vial of saline was confirmed at the time of use by bacterial
culture.

These results support the need for a minimum culture incubation period of 13 days to be applied to both aerobic and anaerobic culture media for all periprosthetic specimens. Optimal recovery of infecting organisms from PJI specimens will be an important component in generating a universal definition for PJI due to indolent agents of infection, such as P. acnes.

clinical microbiology prosthetic joint propionibacterium stomacher 80

Development and Validation of a Quantitative Real-Time PCR Assay Using Fluorescence Resonance Energy Transfer Technology for Detection of Aspergillus fumigatus in Experimental Invasive Pulmonary Aspergillosis

Posted on 2013 by stuartray

Lung tissue in each individual rabbit was sampled and cultured by excision of a representative region of the lung. Each fragment was weighed individually, placed in sterile stomacher® bags, and homogenized with sterile saline for 15 s per tissue sample in a Stomacher® 80.

clinical microbiology pulmonary lung aspergillus fumigatus stomacher 80

How Effective is Sponge Sampling for Removing Bacteria from Beef Carcasses?

Posted on 2013 by stuartray

The Stomacher® 400 was used to blend blend tissue for two minutes as part of the study. The study found that sponge sampling is convenient, however, is a highly variable means of removing bacteria.

food microbiology beef stomacher 400

Regulation of breast cancer stem cell activity by signaling through the notch 4 receptor

Posted on 2013 by stuartray

The Stomacher® 80 was used to blend solid tumors for 12 minutes.

Notch receptor signaling pathways play an important role not only in normal breast development but also in breast cancer development and progression. We assessed the role of Notch receptors in stem cell activity in breast cancer cell lines and nine primary human tumor samples. Our findings suggest that Notch4-targeted therapies will be more effective than targeting Notch1 in suppressing breast cancer recurrence, as it is initiated by breast cancer stem cells.

clinical cytology oncology breast cancer stomacher 80