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Polymerase chain reaction (pcr) for the detection of salmonella in artificially

Thirty-two samples of chicken meat were used. From each sample, 25 grams were weighted in  Stomacher® 400 sterile bags and inoculated with 1 mL of a dilution (10-7,10-8 or 10-9) of S. Enteritidis or S. Typhimurium and 1 mL of the other 18 bacteria pool diluted 10-2 . In two of the samples, only the pool was inoculated. Finally, 225 mL of PBW 1% were added and homogenized in a Stomacher 400 for 30 seconds.

As the results are obtained in a shorter time period than that of microbiological culture, this procedure will be useful in the methodology for detection of Salmonella in chicken.

food microbiology PCR chicken salmonella stomacher 400