Human wharton’s jelly cells-isolation and characterization in different growth conditions

The enzyme solution with tissue is taken out and put in the stomacher strainer bags with inner tissue filter at the rate of 25 ml of enzyme solution per bag. The air in the bag is removed to prevent the bag from rupturing and the bag is sealed at least twice at the top using a heat sealer. The bag is placed in the stomacher 400 Circulator and the stomacher is run at 150 rpm for 10 minutes at 37˚C.

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