Polymerase chain reaction (pcr) for the detection of salmonella in artificially

Thirty-two samples of chicken meat were used. From each sample, 25 grams were weighted in  Stomacher® 400 sterile bags and inoculated with 1 mL of a dilution (10-7,10-8 or 10-9) of S. Enteritidis or S. Typhimurium and 1 mL of the other 18 bacteria pool diluted 10-2 . In two of the samples, only the pool was inoculated. Finally, 225 mL of PBW 1% were added and homogenized in a Stomacher 400 for 30 seconds.

As the results are obtained in a shorter time period than that of microbiological culture, this procedure will be useful in the methodology for detection of Salmonella in chicken.

food microbiology PCR chicken salmonella stomacher 400

Effect of refrigeration and frozen storage on the campylobacter jejuni recovery

The 25-g aliquots were placed in Stomacher® 400 filter bags containing 225 ml of buffered peptone water (HiMedia Laboratories, Mumbai, India). The samples and diluent were mixed in Stomacher® 400 for 60 seconds.

The study found that there was no difference for the recovery of C. jejuni from fresh, refrigerated or frozen samples after selective enrichment, showing that this microorganism can survive under the tested storage conditions.

food microbiology chicken campylobacter jejuni stomacher 400