A Stomacher® 400 was used as part of the study to homogenize beef tissue. The study showed that Baader processing can improve quality and add value to beef shanks by removing the sinew and increasing the lean percentage of the desinewed lean. Processed parts of the shank are more valuable than the whole shank due to sinew now being a legal sausage ingredient.
Meat samples (10 g) were blended in a Stomacher® 400 for 90 s in 90 ml of 0.1% (w/v) peptone water.
The high microbial loads found in this study suggest an improvement of the microbiological quality of retail ostrich meat is convenient.
Sterile meat was thawed rapidly (in about 5 min) by submerging the package in a
50°C water bath, inoculated with enough cells ( 10 ml/100g of meat) for a final
population of ca. 109 stationary- phase cells per g, and stomached for 90 s in
sterile no.400 polyethylene stomacher bags using a Stomacher® 400.
To enumerate L. monocytogenes, 0.1 M phosphate buffer was added to each
stomacher bag (5-g beef slice in 45 ml of phosphate buffer), and the contents were mixed in a Stomacher 400 for 2 minutes.
Thirty-two samples of chicken meat were used. From each sample, 25 grams were weighted in Stomacher® 400 sterile bags and inoculated with 1 mL of a dilution (10-7,10-8 or 10-9) of S. Enteritidis or S. Typhimurium and 1 mL of the other 18 bacteria pool diluted 10-2 . In two of the samples, only the pool was inoculated. Finally, 225 mL of PBW 1% were added and homogenized in a Stomacher 400 for 30 seconds.
As the results are obtained in a shorter time period than that of microbiological culture, this procedure will be useful in the methodology for detection of Salmonella in chicken.
A Stomacher® 400 was used to blend chicken as part of the study. It was used as sample preparation as well as for the enumeration of living bacteria.
The Stomacher® 400 was used to blend reindeer meat for two minutes.
The study found that at slaughter, there was no difference in ASAT activity, urea and Cortisol concentrations between the two transported groups. However, the plasma ASAT activity and urea concentrations at slaughter were significantly lower in the non-transported group. In both transport groups, the plasma Cortisol concentrations increased during loading onto and unloading from the lorry. Abomasal lesions were observed in all treatment groups. It was concluded that reindeer showed an acute stress response to manual handling and transport.
Stomaching, homogenizing, or stomaching followed by homogenizing lettuce treated with sanitizers resulted in recovery of similar numbers of L. monocytogenes, indicating that stomaching and homogenizing are equivalent in extracting cells.
The enzyme solution with tissue is taken out and put in the stomacher strainer bags with inner tissue filter at the rate of 25 ml of enzyme solution per bag. The air in the bag is removed to prevent the bag from rupturing and the bag is sealed at least twice at the top using a heat sealer. The bag is placed in the stomacher 400 Circulator and the stomacher is run at 150 rpm for 10 minutes at 37˚C.
The Stomacher® 80 was used to test tissue from mice. Place the Stomacher bag into the Stomacher, and run the Stomacher on high speed for 10 minutes. Mix the homogenate in the Stomacher bag.